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A Sexual spore production is a characteristic of the Ascomycotina severe gastritis diet plan generic omeprazole 20 mg with amex, which produce an ascus (saclike structure) after the union of two nuclei gastritis chest pain buy generic omeprazole 10 mg. All of these options Microbiology/Apply knowledge of fundamental biological characteristics/Mycology/2 16. It is usually considered a contaminant but is an opportunistic pathogen, and must be identified when found in specimens from immunosuppressed patients. A the yeast isolate is inoculated directly into the molten agar base free of carbohydrates or is poured as a suspension onto a yeast nitrogen agar base plate. Growth around the disk indicates the ability of the yeast to utilize the carbohydrate(s) as a sole source of carbon. A positive germ tube is a presumptive identification along with the production of blastoconidia, terminal chlamydospores, and pseudohyphae. Carbohydrate assimilation tests are used for the identification of yeast isolates by inoculating media: A. Containing yeast extract Microbiology/Apply principles of basic laboratory procedures/Mycology/1 19. No further testing is needed for identification Microbiology/Select course of action/Mycology/3 7. A blood agar plate inoculated with sputum from 449 a patient with diabetes mellitus grew very few bacterial flora and a predominance of yeast. Given the following results, what is the most likely identification of the yeast isolate Histoplasma capsulatum Microbiology/Apply knowledge of fundamental biological characteristics/Mycology/2 A. Latent Microbiology/Apply knowledge of fundamental biological characteristics/Mycology/1 21. The mycelial form of which dimorphic mold produces thick-walled, rectangular, or barrel-shaped alternate arthroconidia Blastomyces dermatitidis Microbiology/Apply knowledge of fundamental biological characteristics/Mycology/2 as oval or elongated cigar shapes Blastomyces dermatitidis Microbiology/Apply knowledge of fundamental biological characteristics/Mycology/2 on agar resembles: A. Microbiology/Apply knowledge of fundamental biological characteristics/Mycology/2 22. Histoplasmosis is a chronic granulomatous infection primarily found in the lungs that invades the reticuloendothelial system. Infection occurs via spores released from decaying bird or chicken droppings that are inhaled when disturbed. Which group of molds can be ruled out when septate hyphae are observed in a culture Both display aseptate hyphae, while the other groups above display septate hyphae. Zygomycetes usually not encountered in clinical specimens are also aseptate and include Absidia spp. Microbiology/Apply knowledge of fundamental biological characteristics/Mycology/1 28.
One or two bands are seen for each locus correlating to reactivity with a specific antigen or group of antigens B gastritis symptoms chronic order omeprazole 10 mg with visa. Each well contains a primer to a specific base sequence of one allele or allele group gastritis diet 7 hari buy omeprazole 20mg cheap. Bands are stained with ethidium bromide and can be visualized by direct observation with a near ultraviolet light source. B the -globin gene is located near the end of the short arm on chromosome 11 and consists of three exons and two introns constituting 1,600 base pairs. This causes production of a fragment that is 200 base pairs longer than seen for the normal -gene. Heterozygotes produce one normal and one longer band, whereas homozygotes produce a single band that is 200 base pairs longer than the normal amplicon. Which of the following genetic diseases is caused 517 by an expanded trinucleotide repeat Fragile X is so named because when cells from an affected individual are cultured in folate-deficient medium, the long arm of the X chromosome appears to have a break caused by deficient staining. In fragile X syndrome, the repeat is extended and its length determines whether the affected persons will show mental retardation. The gene is needed for normal brain function and its underexpression results in mental retardation. Females in whom the premutation expands in size to a full mutation transmit the syndrome to all of their male and half of their female offspring. The size of the fragments and their fluorescence determine which alleles are present. A single exclusion can result from germ line mutation within one locus being tested C. The biological mother may be different than the purported mother Molecular/Apply knowledge of special procedures/ Paternity testing/3 type of mutation Single nucleotide substitution Molecular/Correlate clinical and laboratory data/Point mutations/2 oncogene Hemoglobin S/-thalassemia Molecular/Apply knowledge of special procedures/ Thalassemia/2 hemoglobin C gene is present in fetal cells B Two exclusions are needed rather than one to be 100% certain of nonpaternity because of the rare possibility of a mutation having occurred in one of the loci being tested. Exclusion is based on the premise that the biological father must have at least one allele in common with the child at each locus. D Hereditary hemochromatosis is an autosomal recessive disease with a frequency as high as 0. The remaining cases are caused by a single-point mutation at position 63 on the protein (H63D), which produces a milder increase in iron absorption. A -Thalassemia carriers have a full or partial deletion of one or two of their four globin genes. Selecting the appropriate counting aperture Molecular diagnostics/Apply principles of special procedures/Flow cytometry/1 31. Which of the following parameters are used to gate subpopulations based upon their light-scattering properties. Cells to be interrogated by the laser(s) are selected by identifying the area in which they appear on a scatterplot. B the gated population is selected by evaluating the scatterplot of forward light scattering (x axis) and right angular or side scatter (y axis). For example, monocytes can be differentiated from neutrophils because the former have greater forward scatter and less side scatter. Impedance amplitude versus background conductance Molecular diagnostics/Apply principles of special procedures/Flow cytometry/1 32. A Forward scatter of light from a laser directed through the aperture of the cytometer is directly related to cell size. Right angular scatter (side scatter) is dependent upon the number of granules inside the cytoplasm. For example, small lymphocytes that are agranular have the lowest forward and side scatter and are easily identified as the cluster of cells closest to the bottom and left of the scatterplot. In general, which statement best characterizes the relationship between white blood cells and light scattering in flow cytometry Forward scatter is inversely related to size and side scatter is directly related to size D.
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- Detoxification is the withdrawal of an abused substance in a controlled environment. Sometimes a drug with a similar action is taken instead, to reduce the side effects and risks of withdrawal. Detoxification can be done on an inpatient or outpatient basis.
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